Rapid tagging and integration of genes in Giardia intestinalis.

نویسندگان

  • Stéphane Gourguechon
  • W Zacheus Cande
چکیده

We developed a series of plasmids that allow C-terminal tagging of any gene in its endogenous locus in Giardia intestinalis, with different epitope tags (triple hemagglutinin [3HA] and triple Myc [3Myc]) and selection markers (puromycin, neomycin, and a newly developed marker, blasticidin). Using these vectors, cyclin B and aurora kinase were tagged, expressed, and localized.

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عنوان ژورنال:
  • Eukaryotic cell

دوره 10 1  شماره 

صفحات  -

تاریخ انتشار 2011